CD4 antibody (Non-Polymorphic Site) (PE)

Catalog No. ABIN118165

This Rat Monoclonal antibody specifically detects CD4 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD4 antibody (Non-Polymorphic Site) (PE) (ABIN118165)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: YTS177-9

Product Details anti-CD4 Antibody (PE)

Binding Specificity: Non-Polymorphic Site

Specificity: This antibody reacts with the CD4 antigen, non polymorphic epitope. The antibody is reported to block MHC class II dependant T-cell responses in vitro and in vivo and induces tolerance. We recommend SM019A for such studies.

Purification: Affinity chromatography on Protein G

Immunogen: Mouse spleen cells. Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL (SM019R) or 0.25 (SM019RT) distilled water.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE! Protect from light!

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 12504

NCBI Accession: NP_038516

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events